Food Analysis: Starch, Protein and Reducing Sugars

victuals analysis Starch, Protein and Reducing SugarsA soft and Quantitative study of Food Analysis to test for the social movement of amylum, protein and for trim down sugars.Sorina PopaLaboratory Partner Chris PatersonObjectives The aims for this sample are to carry out a number of qualitative procedures in order to identify antithetical diet traces such as amylum, protein and glucose that are play or absent in spite of appearance the aqueous tests.IntroductionFood science is the application of biological, chemical and strong-arm composition of sustenance as swell up as the concepts that highlight fodder processing. The governance deep down the Food Science Industries are exceptionally relentless when it comes to the quality and the safety of their manufactu blushing(a) products. The role of a Food Scientist is to view the microbiological, biochemical and physical equities of food. The main three categories of food are proteins fats and carbohydrates. benedicts test was considered perfect for this taste as this test is throw out detect a appointed prove for reducing monosaccharides or disaccharides sugars such as glucose and lactose. During the process of heating, the alkali in the Benedicts reagent becomes super reactive and it behaves as a strong reducing agent.The signifi suffert people of color alternate that occurs from a clear blue colour to a brick red precipitate insinuates a positive forget for the carriage of glucose at heart a food sample. The green/yellow colour interchange can prefigure a vague comportment of protein as well as the comportment of glucose.Starch is an example of a polysaccharide that is found mostly within plants. Plants use starch to store energy.The atomic number 53 test is extremely untoughened and can detect up to 0.00002M of a theme well-tried at a temperature of 20 C. However, if the samples pH is low, the iodine resolves reactions wouldnt occur with the sample reply tested. iodine event determines the nominal head of starch most easily when the cell walls are modify and broken down in order to chemically react with the starch grains. In the comportment of starch on that point is a colour change from embrownness to benighted/Black. It is anticipate for starch to be present within potatoes, flour, certain vegetables and cereals.Proteina and amino acids are the building blocks of protein. The amino acids are conjugated by peptide bonds. Protein is highly found within meat, seeds and beans.The biuret test was considered ideal for this experiment as it can detect higher concentrations of protein.The reaction formed comes from an constitutive(a) compound as its heated and a high temperature outleting to a positive test for protein. The biuret reagent is composed of diluted copper sulphate within a strong alkali.The colour change from blue to purple originates from the coordinate mingled that is found between the cupric ions and the nitrogen atoms.The Bradford Test was also considered for this experiment as it its a rapid and highly sensitive method for scrutiny for Protein. 1Materials The materials were as in the ITT Biology1 Food Analysis Protocols Manual. 2Methods There were 4 various experiments carried out in order to test for different components withing different food samples tested. The Iodine Test, Benedict Test, Biuret Test and Bradford Test were carried out as per Itt Biology1 Food Analysis Protocols Manual. 2Results/DataTable1 bank check Results indicating the presence of reducing sugars applying Benedict resolve on the different aqueous outcomes tested in the research lab 6Table2 tab Results indication the presence of Starch applying Iodine solution on the different aqueous solutions tested in the laboratory 5Table 3 Assay Results indication the presence of Protein applying Biuret Reagent on the different aqueous solutions tested in the laboratory 3Table 4 Assay Results indication the presence of Protein appl ying Bradford Reagent on the different aqueous solutions tested in the laboratory 4DiscussionThe next table will indicate the expected results of the solutions tested in the laboratory 7 resultant role A From the data obtained thither is a positive result for the presence of glucose as the colour obtained was Green. The colour Green shows that there was other trace component as well as the presence of Glucose. For the Iodine test the colour obtained was Yellow towards embrown which indicates there was a blackball result for the presence of starch. There was a negative result for the presence of protein apply the Biuret Reagent as the original colour unappeasable remained the same. However, when this solution was tested for the presence of protein using Bradford Reagent, there was a colour change from Brown to Light mordant indicating that there are small traces of protein present within this solution. This leads to a expiration that solution A can be class as being High En ergy Glucose Drink diluted 1 in 20. etymon B From the data obtained it showed to have the presence of Glucose as the colour obtained was Green. The colour Green tells us that traces of protein has been present as well as Glucose. To sustain this statement, when solution B was tested using Bradford Reagent , it showed a positive result for the presence of protein due to the colour change from Brown to Blue. However there was no presence of Starch within this solution as the colour remained the iodine brown/yellow original colour. This leads to the conclusion that solution B might have been the nutriment change stinker Lime Drink diluted 1 in 20. dissolving agent C Looking at the data obtained, Reducing sugars are present within this solution as the colour changed from Blue to Green. The Iodine test showed a negative result for the presence of Starch as the colour remained Brown. However, traces of Protein was present when the Bradford Test was carried out. This signifies that the solution C can be the Carbonated Diet Lemon and Lime Drink diluted 1 in 1000.According to the expected results, the Carbonated Lemon and Lime Drink diluted 1 in 1000 showed to have a positive result for Reducing sugar, and Protein. However, since this solution was diluted 1 in 1000, its possible that the presence of protein didnt show because of the insufficient amount of protein. The Bradford Test showed to have the ability to indicate small traces of protein. firmness D Analysing the data obtained the wasnt either presence of Glucose in the Reducing Sugars Assay as the colour remained blue. There wasnt any Starch present when Iodine solution was added in the root word D due to no change in the colour i.e. Brown. However, the Biuret Test indicated a positive result for the presence of protein due to the colour change from Blue to Purple. As the Biuret test is known to detect large traces of protein, the presence of protein also came positive when the Bradford Reagent was used. Lo oking at the different solutions and their expected results, Solution D can be considered to be Sports Supplement diluted 1 in 10 since it is said its based on protein and non so much Carbohydrate based. This can be the reason why the Iodine and Benedict Test showed negative results.Solution E From the results obtained it indicated that there are traces of Glucose as the colour changed from Blue to Yellow/Brown. The colour brown isnt the presence only for the presence of glucose which is orange. There are traces of a different component within the Solution E. There arent any traces of Starch when the Iodine Solution was added to a sample of Solution E. However, the redundant trace element that appeared during the Bradford Test.The colour changed from Brown to dark-skinned Blue signifying traces of protein. When the solution was tested for protein using the Biuret Reagent, it showed a negative result as there was no colour change. As a conclusion, the Solution E appears to be Cows Milk diluted 1 in 20.Solution F Analysing the data obtained from carrying out different aqueous procedures, it showed that there is a negative result for the presence of Reducing Sugars as the colour remained Blue after boiling process. There was also a negative result for the presence of Starch when Iodine solution was added and the colour remained the colour of Iodine solution i.e. Brown/Yellow. However, there was a positive result using both Biuret and Bradford Reagents as the colour changes to Purple when Biuret Reagent was used and the colour changed to dark Blue when Bradford Reagent was used. Comparing the result from this solution to the other expected solutions, Solution F can be looked upon to be Liquidised Cauliflower Filtered and Diluted 1 in 5.Solution G From the results obtained it showed that there was a positive result for the presence of Glucose as there was a colour change from Blue to Brown. The colour brown also indicates there is another trace element present w ithin this solution as well as Glucose. In the Iodine Test there was a positive result for the presence of starch as the colour change was Orange. This is an unusual colour as the actual positive colour indication for the presence of starch is Blue/Black. However, the colour orange can indicate there are more than than one trace elements that are present within this solution. When the Bradford Test was carried out, a negative result was obtained as the colour did not change in Biuret and Bradford Test. From the data obtained, Solution G can be Diet Carbonated Lemon and Lime Drink diluted 1 in 10.Solution H From the results obtained it showed a negative result for reducing sugar as the colour remained Blue. However, there was a positive result for the presence of starch as the colour changed from Brown/yellow to Orange. There was also a positive result when the solution was tested for protein. When Biuret Reagent was used the colour didnt change which leads to a negative result but when the Bradford Reagent was used there was a positive result as the colour changed into Light Blue. This can mean that the Solution H can be classified to be Wheat flour 0.5% w/v solution.ConclusionThe purpose of these soft experiments was to analyse in depth the aqueous solutions given and tested for the presence of starch, traces of protein and for reducing sugars. The data collected from each solution was compared with the expected results from particular(prenominal) solutions from which some were diluted more than 5 times. The results obtained were remarkably close to the results expected. This concludes that the boilersuit assays undertaken were a success due to the results obtained and discussions made.ReferencesBooks/Manuals1 ITT Laboratory Skills Manual (2014-2015), Biology1, pg. 44-512 ITT Biology Analysis Protocols (2014-2015)3 Malhotra VK (2003) 4th Edition, Food Analysis, Practical Biochemistry for Students, Ch.48.4 Shanmugam S., Sathish Kumar T, Panneer Selvam K, (2 010) Laboratory Handbook on Biochemistry, east Economy Edition, pg26-27Online Links5 British Nutrition Foundation (2004), Test for Starch, (Energy and Nutrients1995) (http//www.foodafactoflife.org.uk/attachments/92592385-dfb2-4ad6d61cf053.pdf) Accessed on 26th October 2014 10.00am.6 British Nutrition Foundation (2004), (Energy and Nutrients 1995) (http//www.foodafactoflife.org.uk/attachments/f4525d5b-0133-49b1e394c729.pdf) Accessed on 26th October emailprotected 10.30am.7 Conde Nast (2014) ego Nutrition Data (http//nutritiondata.self.com/facts/beverages/7399/2) accessed on 26th emailprotected.